The purpose of this study was to determine nutrient values, bacteria and fungus flora of 60 mixed feed samples obtained from Diyarbakir province. The nutrient values, bacteria and fungus flora of all feeds were analyzed by classical methods. In the mixed feeds of dairy cows, beef cattle, calf and lamb; dry matter, crude protein, crude fat, crude fiber, nitrogen free extract matter and crude ash differences among the feeds were not statistically significant (p>0.05). In this research, crude fiber levels have found higher than standard values in calf and lamb feeds. However, in the mixed dairy cows, calf and lamb feeds, crude protein values were lower than standard values. While the highest total mesophilic aerobe bacteria counts were found in lamb feeds, the lowest were in dairy cow feeds. The highest values of mould and yeast were determined in beef cattle feeds. Although Salmonella were not detected in beef cattle, calf and lamb feeds, in dairy cow feeds, they were found 13.33 %. The contamination rates were found as 13.33 %, 46.66 %, 33.33 %,13.33 % for Listeria monocytogenes; 93.33 %, 86.66 %, 93.33 %, 80.00 % for coliform bacteria; 40.00 %, 26.66 %, 20.00 %, 40.00 % for Escherichia coli; 86.66 %, 80.00 %, 93.33 %, 60.00 % for Staphylococcus spp.; 73.33 %, 73.33 %, 60.00 %, 46.66 % for sulfite reducing anaerobe bacteria; 100.00 %, 100.00 %, 86.66 %, 93.33 % for Bacillus cereus; 93.33 %, 80.00 %, 100.00 %, 86.66 % for mould and 80.00 %, 73.33 %, 100.00 % and 80.00 % for yeast in dairy cows, beef cattle, calf and lamb feeds, respectively. As a result, in the mixed feeds of dairy cows, calf and lamb used in ruminant nutrition in Diyarbakir province, both microbiological and nutrient quality were low, however in beef cattle feeds, only microbiological quality was low.