In this retrospective study, nuclear morphometric parameters of histopathologically confirmed anal sac gland adenocarcinomas were investigated on cytological smears in dogs and compared with the neoplastic subtype. The correlation between morphometric parameters and histopathological subtype was determined using Mann-Whitney U test (Statistica 6.0, StatSoft, USA) at level of significance P<0.05. There were statistically significant differences between all studied morphometric parameters of tubular, rosette and solid anal adenocarcinoma patterns. The results of this preliminary study indicated that nuclear morphometry could be used as an auxiliary method for subtyping canine anal sac gland adenocarcinomas.