Indigenous chickens play an essential role in the Iranian economy especially in the rural sector. Juxtaposing the economic factor against the state of the health of the indigenous birds in Iran, more specifically the prevalence of parasitic infectious diseases, this study was conducted to determine the presence and intensity of species of helminthes in the alimentary canals of the local chickens in the Isfahan area, which is centrally located in Iran. For the purpose of this study, 100 local chickens from five different parts of the geographic region of Isfahan were collected randomly, during 2010-2011. The chickens were slaughtered and their gastrointestinal tracts and alimentary canals where examined for the presence of helminthes. The study identified three nematodes, and two cestodes species. No trematodes were found. From the 100 local chickens examined, 81 were infected (81%). The distribution of the presence of species was as follows: Raillietina tetragona (46%), Heterakis gallinarum (42%), Ascaridia galli (36%), Raillietina echinibothrida (7%) and Subulura brumpti (2%). More research is needed to identify all parasites in the region, and to understand the causes of infection, disease and death. Such research will provide the basis for improving the conditions including a reduction of the high mortality rate of local chickens in the area. It is therefore necessary that periodical parasitological investigations as well as anthelmintic treatments should be carried out regularly.