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A total of 32 bitches were used in this study, out of these 16 bitches were inseminated with fresh semen and the other with semen frozen by paillette technique in 4% glycerol containing Tris-fructose-citric acid extender by intravaginal route. The optimal insemination time was determined by Ovulation Test Kit and inseminations were repeated in both groups every other day for 3 times considering the semi-quantitative progesterone value (1-8 ng/ml). Pregnancy rate was 81.25% for the fresh semen group and 37.50% for the frozen semen group. Pregnancy rates in the fresh semen group have been significantly higher than the frozen semen group (p<0.01). Mean litter sizes per birth in the fresh and frozen semen groups have been 4.69 and 3.33, respectively.
At the end of the study, it is concluded that intravaginal artificial insemination with fresh semen has the advantages not to need anesthesia or chirurgical applications and can be accomplished by every pet veterinarian practically, on the other hand no satisfying pregnancy rate could be achieved with frozen semen.