Acta Veterinaria Eurasia
Research Article

EFFECTS OF SUPPLEMENTS TO MATURATION AND FERTILIZATION MEDIA AND CO-CULTURE WITH SHEEP OVIDUCTAL EPITHELIAL CELLS ON IN VITRO PRODUCTION OF SHEEP EMBRYOS

1.

Istanbul University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination

2.

Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul University, 34320, Avcilar, Istanbul, TURKEY

3.

İstanbul Üniversitesi Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı 34320, Avcılar, İstanbul

4.

İstanbul Üniversitesi Veteriner Fakültesi Dölerme ve Sun 'i Tohumlama Anabilim Dalı, 34320 Avcılar, İstanbul

Acta Vet Eurasia 2003; 29: 157-165
Read: 766 Downloads: 540 Published: 26 December 2019

In this study in which the effects of maturation and fertilization media supplements and co-culturing on in vitro production of sheep embryos were investigated, primary oocytes (n=454) collected from ovaries of slaughtered ewes were used. Oocytes divided randomly into 4 groups were matured for 26 h in TCM 199 medium with different supplements:

Group I (SH): TCM 199 medium supplemented by 20% sheep estrous serum, 10 ug/ml FSH, 10 ug/ml LH and 1 ug/ml estradiol 17fi,

Group (S): TCM 199 medium supplemented by 20% sheep estrous serum.

Group 3 (H): TCM 199 medium supplemented by 10 ug/ml FSH, 10 ug/ml LH and 1 pg/ml estradiol

170, and

Group 4 (M): TCM 199 medium without serum and hormones.

After maturation, oocytes which were again divided into 3 subgroups were transferred in SOF medium supplemented by 2% (D), 5% (O) and 10% (Y) sheep estrous serum and fertilized in vitro for 18 h with fresh ram semen collected by electro-ejaculation and washed. After fertilization, oocytes were divided into 2 subgroups [Group 1 (KK): Co-culture with sheep oviductal epithelial cells; Group 2 (K): Culture without sheep oviductal epithelial cells] and cultured for 7 days. At the end of this period embryos were evaluated for their developments.

The highest cleavage and morula rates were obtained in SH+Y+KK group (62.5% and 43.8%, respectively) and the lowest rates (8.7% and 4.3%, respectively) in M+D+K group in this study.

According to the results, we concluded that supplementation of in vitro maturation media with 20% sheep estrous serum and hormones, in vitro fertilization media with 10% sheep estrous serum and co-culturing with sheep oviductal epithelial cells after fertilization could improve the cleavage and morula rates.

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EISSN 2619-905X