Acta Veterinaria Eurasia
Original Article

Docosahexaenoic and Oleic Acid Dissimilarly Modulate Lipid Metabolism of Immature and Already Mature Adipocytes in Vitro


Department of Animal Physiology, Trakia University, Faculty of Veterinary Medicine, Stara Zagora, Bulgaria


Department of Chemistry and Biochemistry, Trakia University, Faculty of Medicine, Stara Zagora, Bulgaria

Acta Vet Eurasia 2023; 49: 19-25
DOI: 10.5152/actavet.2022.21121
Read: 796 Downloads: 334 Published: 05 December 2022

The study aimed to compare the effect of 100 μM docosahexaenoic acid and oleic acid alone, and as a combination in immature and already mature 3T3-L1. The cells were subjected to adipogenic induction for 2 days and then were maintained in a high glucose culture medium supplemented with 10 μg/mL of insulin for additional 16 days. The treatment with docosahexaenoic acid and oleic acid lasted 9 days in total, as half of the cells were treated during induction (from day 1 to 9), and the rest—after reaching maturity (day 9–18). The palmitic fatty acid was used as a positive control. Applied in immature adipocytes, docosahexaenoic acid prevented intracellular neutral lipid deposition and reduced lipolysis upon well-preserved glucose utilization. At the same time, oleic acid alone or combined with docosahexaenoic acid increased lipid accumulation and slightly attenuated lipolysis only related to the palmitic fatty acid-treated group. However, free fatty acids application in mature adipocytes did not cause any beneficial effect. They elevated lipolysis rate compared to the induced control, which in vivo might be a predisposing factor for ectopic fat accumulation. The results emphasized that free fatty acids’ impact on adipocyte metabolism was highly affected by their maturation stage and should be considered in anti-obesity strategies based on free fatty acids signaling modulations.

Cite this article as: Ivanova, Zh., Grigorova, N., Vachkova, E., Tacheva, T., & Georgiev, I. P., (2022). Docosahexaenoic and oleic acid dissimilarly modulate lipid metabolism of immature and already mature adipocytes in vitro. Acta Veterinaria Eurasia, 49(1), 19-25.

EISSN 2619-905X