Acta Veterinaria Eurasia
Research Article

Detection of Single Nucleotide Polymorphism in the 5'-Untranslated Region (5'UTR) of Lactoferrin Gene and Its Association with Clinical Mastitis in Cattle

1.

PhD Student at Department of Clinical Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran

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Department of Clinical Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran

Acta Vet Eurasia 2014; 40: 131-138
Read: 232 Downloads: 154 Published: 23 December 2019

Lactoferrin (Lf), plays an important role in the innate immune system and defence mechanism of mammary gland of milk producing animals. The amount of lactoferrin increases during inflammatory process and viral infections. Many polymorphisms have been found in the bovine Lf Gene. The present study aimed to identify single-nucleotide polymorphic (SNP) site in the 5' untranslated region (5'-UTR, position +32) of the bovine Lf gene in Holstein dairy cattle and to quantify their association with clinical mastitis using Allele-specific polymerase chain reaction (PCR) method. Blood samples were collected from 89 multiparous Holstein dairy cows with a history clinical mastitis (n=38), and cows without mastitis as control group (n= 51). In this study, we detected type of SNP in the 5' untranslated region (5'-UTR, position +32) using a designed Allele specific PCR that validated following sequencing method. We did not found evidence for a significant association between SNP in the 5'-UTR (+32: G/G) Lf gene and the mastitis in dairy cattle in Iran. The results of this study did not provide any support to considered 5'-UTR polymorphism (position +32) as a marker for resistance/susceptibility to mastitis in dairy cattle.

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ISSN 2618-639X EISSN 2619-905X