Detection Of Salmonella Enteritidis Infections In Poultry By Using Bacteriological And Serological Methods

In this study the presence of Salmonella Enteritidis was investigated by isolation from broiler chickens in Aydın and the presence of specific antibody on blood sera samples from the same broilers were also comparatively evaluated by Enzyme-Linked Immunosorbent Assay (ELISA) and Serum Plate Agglutination Test (LAT). The materials of the study were cloacal swaps and blood samples taken from 460 live, unvaccinated broiler chickens aged between 22 to 37 days from 15 poultry farms. Nineteen (4.1 %) Salmonella Enteritidis isolations were achieved. The susceptibility of isolates against 8 antibiotics was determined by standard disc diffusion test. Out of 19 strains, 19 (% 100.0), 19 (% 100.0), 12 (63.2), 9 (% 47.4) of the strains were resistant to penicilline G, erythromycine, gentamycine, ampicilline; 13 (% 68.4), 13 (% 68.4), 12 (% 63.2), 8 (% 42. 1) of the strains were sensitive to danofloxacin, trimetophrimsulfamethoxazole, tetracycline, kanamycin respectively. In blood sera 56 (12.2 %) and 109 (23.7 %) seropositiveness were determined by LAT and ELISA. In conclusion, it was revealed that Salmonella Enteritidis generates a potential risk for both poultry and human health in Aydın