Acta Veterinaria Eurasia
Original Article

Prevalence and Antibiotic Resistance of Thermotolerant Campylobacter spp. in Chicken Meat Sold in İstanbul


İstanbul Arel Üniversitesi Sağlık Bilimleri Yüksekokulu Beslenme ve Diyetetik Bölümü, 34537 Tepekent, Büyükçekmece, İstanbul, Türkiye

Acta Vet Eurasia 2017; 43: 98-109
DOI: 10.16988/iuvfd.282027
Read: 701 Downloads: 357 Published: 22 December 2019

Campylobacter spp. are some of the most common causes of bacterial diarrhea in humans worldwide. They are mainly considered as foodborne pathogens that are found in raw or undercooked poultry and serve as an important source of sporadic campylobacteriosis. The present study was aimed to determine the prevalence and the antimicrobial resistance patterns of thermotolerant Campylobacter spp. in chicken meat. A total of 176 samples of chicken meat were analyzed using PCM and BAX® system. The samples analyzed included: 56 samples of whole chicken, 27 samples of chicken breast, 33 samples of chicken thigh, 25 samples of chicken drumstick and 35 samples of chicken wings. Samples of all the fresh chicken meat sold in İstanbul were randomly purchased from different major supermarkets in their original, individual packages. Laboratory analyses to detect thermotolerant Campylobacter spp. were performed in accordance with the ISO 10272-1, 2006 standard (qualitative analysis). API® Campy (BioMerieux, Marcy-l’Etoile, France) was used for the confirmation of presumptive colonies. Campylobacter isolates were subjected to antimicrobial susceptibility tests by the disc diffusion method as recommended by the National Committee for Clinical Laboratory Standards. Zones of growth inhibition were evaluated according to the NCCLS standards. Using PCM, the prevalence of C. coli, C. jejuni and C. lari was determined as 15.34, 8.52 and 1.7%, respectively. However, using BAX® system, the prevalence was determined as 15.90, 18.75 and 1.7% for C. coli, C. jejuni and C. lari, respectively. C. coli was resistant to nalidixic acid (78.57%), ofloxacin (14.29%) norfloxacin (10.71%) and ampicillin (10.71%). But the highest resistance was observed to nalidixic acid (90.91%) for C. jejuni and (100%) for C. lari. In conclusion, considering the public health, chicken meat is a common source for Campylobacter strains and antibiotics should be used carefully in veterinary medicine.

ISSN 2618-639X EISSN 2619-905X