Acta Veterinaria Eurasia
Original Article

Possible Role of Hypoxia-Inducible Factor-1Alpha and Vascular Endothelial Growth Factor During Periovulation and Corpus Luteum Formation in Cows

1.

Animal Biotechnology, Faculty of Agriculture and Veterinary, University of Prishtina, Prishtina, Kosovo

2.

Academy of Science of Albania, Tirana, Albania

3.

Animal Physiology and Immunology, Weihenstephan, Technical University of Munich, Weihenstephan, Germany

Acta Vet Eurasia 2022; 48: 211-215
DOI: 10.5152/actavet.2022.22027
Read: 88 Downloads: 35 Published: 01 September 2022

The objective of the study was to characterize expression patterns of hypoxia-inducible factor-1alpha and vascular endothelial growth factor isoforms during periovulation in the cow. Ovaries containing follicles or corpora lutea were collected by transvaginal ovariectomy (n=5 cows/group) and timely defined (after gonadotropin-releasing hormone application) as follows: (I) 0 h; (II) 4 h; (III) 10 h; (IV) 20 h; (V) 25 h (follicles); and (VI) 60 h (early corpora lutea). The mRNA expression of genes encoding hypoxia-inducible factor-1alpha and vascular endothelial growth factor isoforms was quantified by reverse transcriptase quantitative polymerase chain reaction. The high mRNA expression of hypoxia-inducible factor-1alpha in the control follicle group (before gonadotropin-releasing hormone) was followed by a continuous and significant downregulation afterward with a minimum level in follicle group close to ovulation, and a significant increase just after ovulation. The mRNA of vascular endothelial growth factor isoforms was high during luteinizing hormone peak (4 h after gonadotropin-releasing hormone), decreased significantly thereafter to the lowest levels in follicle group 25 h after gonadotropin-releasing hormone, followed by a rapid and significant increase just after ovulation (newly corpora lutea). In conclusion, our results of hypoxia-inducible factor-1alpha and vascular endothelial growth factor regulation in follicle groups around ovulation indicate them to be necessary mediators of the luteinizing hormone-dependent ovulation and significant molecular mechanisms of angiogenesis during early corpora lutea formation.

Cite this article as: Berisha, B. (2022). Possible role of hypoxia-inducible factor-1alpha and vascular endothelial growth factor during periovulation and corpus luteum formation in cows. Acta Veterinaria Eurasia, 48(3), 211-215.

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