Ozone is a molecule consisting of three oxygen atoms, formed as a result of oxygen being exposed to high-energy electric current and ultraviolet rays in nature. It is a powerful oxidising agent. Therefore, it has bactericidal, virucidal and fungicidal properties. Ozone treatment is a therapy method consisting of mixing pure oxygen and ozone at certain ratios (0.05-5% O3 ; 95-99.95% O2) and administering this combination to the patient via different routes. Ozone has an oxidising effect on the organism and activates the antioxidant enzyme systems. It implements the release of cytokines such as interferons and interleukins by leucocytes and stimulates the immune system. Ozone has 30 different methods of administration which can be classified under main headings such as; intravenous, intramuscular, transdermal, rectal insufflation and inhalation. Ozone treatment is used in wound healing, tumours, peritonitis cases, orthopaedic disorders, eye disorders and dentistry. This review aims to share the therapeutic effects and easy administration of ozone with colleagues and present as a new treatment procedure to veterinary practice in our country.