The present study was to investigate the incidence of Helicobacter pullorum in caecum and colon of 96 broiler chickens. All samples were obtained from different commercial broiler slaughtering facilities in Marmara region of Turkey. The presence of H. pullorum was assessed by conventional culture method and specific polymerase chain reaction (PCR) test. H. pullorum were not isolated from any of the samples. In contrast, H. pullorum DNA was detected by PCR in 55.21% (53/96) of the samples. Also, it was indicated that caecum was commonly infected than colon. The results showed that H. pullorum is present in Marmara region of Turkey. Further studies are needed to identify the risk factors for the presence of H. pullorum for human health.