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Bio-active compounds from marine flora and fauna contain various types of pharmacologically active novel biomolecules enriched with different types of lectins. Lectins, with high saccharide binding specificities have been considered important probe to monitor the changes in plasmamembrance during physiological maturation of spermatozoa. Cytosol fraction, isolated from the spermatheca and / or ovotestis gland of a marine mollusc Telescopium telescopium, specific for sialic acid, has been found responsible for "Head to Head" sperm agglutination, and the agglutinability varies in number (p<0.01) among the epididymal regions. Number of hypo-osmotic swelling-positive spermatozoa also exhibited region wise variation (p<0.01) where both the characters remained maximum in caudal region. The differences in the number of agglutinating spermatozoa and their pattern reflects modification of sperm surface carbohydrate moieties linked either with lipid or protein that occur during epididymal transit and heterogenic nature was due to regional variation in the carbohydrate domain of sperm plasmamembrane. Differences (P<0.01) in the number of hypo-osmotic swelling-positive spermatozoa between the epididymal regions indicated sperm plasmamembrane in the caput are less permeable and less functional than spermatozoa from corpus and caudal segment. These indicated maturation along with alteration of sperm plasmamembrane occurs gradually during epididymal passage. Present approach with this bio-active compound obtained from marine snail could be used as a biomarker to study the sperm maturation process, and heterogeneity and status of the plasmamembrane which might help to improve the diagnosis of reproductive problems in relation to distribution of lectin receptors on sperm cells of any species to predict status of the male gametes before use in artificial reproductive technologies.