Acta Veterinaria Eurasia
Original Article

Effect of Solvents on Phytoconstituents and Antimicrobial Activities of Ocimum gratissimum and Eugenia caryophyllata Extracts on Listeria monocytogenes

1.

Department of Aquaculture and Fisheries, University of Ilorin, Nigeria

2.

Department of Aquaculture and Fisheries Management, University of Ibadan, Nigeria

3.

Department of Veterinary Pathology, University of Ibadan, Nigeria

Acta Vet Eurasia 2018; 44: 31-38
DOI: 10.5152/actavet.2018.007
Read: 178 Downloads: 71 Published: 16 January 2018

This study examined the effect of differentsolvents on active ingredients and antimicrobial performance of fresh leaves ofOcimum gratissimum and dried buds of Eugenia caryophyllata. Plant parts wereextracted in a soxhlet apparatus using dietyl-ether, ethyl acetate, andethanol. Phytoconstituents of the plants was determined using gas chromatography-massspectrometer (GC-MS). The extract with high eugenol concentrations was preparedin seven treatments (160, 80, 40, 20, 10, 5, and 0 mg/mL) and tested againstListeria monocytogenes using agar well diffusion methods in triplicates.Minimum inhibitory concentration (MIC) and zone of inhibition were determinedusing standard technique. The data was subjected to descriptive statistics andone-way analysis of variance (ANOVA) at α0.05. The results showed that the phytochemical concentration of theextract varied significantly (p<0.05) with respect to solvents. Extract ofE. caryophyllata using ethyl acetate had highest concentration of eugenol(71.32%) while O. gratissimum had 16.67%. The results revealed anti-L.monocytogenes activities of fresh leaf of O. gratissimum and dried buds of E.caryophyllata capacity with zones of inhibition of 13.33 mm and 19.00 mmrespectively and MIC of 80 mg/ml. This establishes the possibility of usingfresh leaf of O. gratissimum and dried buds of E. caryophyllata to prevent thegrowth of foodborne bacterias, especially L. monocytogenes.

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ISSN2618-639X EISSN 2619-905X