There have been many studies which investigated the effect of ovariohysterectomy on metabolic activity in bitches. The slowing down effect of ovariohysterectomy on metabolism and it’s results was emphasized by the researchers. The purpose of the presented study is to determine the early term effects of neutering on bodyweight, serum total cholesterol, trigliceride, alkaline phosphatase, alanin aminotranspherase, aspartate aminotranspherase and glucose values in bitches with different breed, age and living conditions. For this purpose serum total cholesterol, triglyceride, glucose levels and body weight of 15 bitches were determined at pre-operative and post-operative 3rd - 6 th and 12th months. When the body weights of different times were considered statistically, the difference between pre and postoperative 6th and pre and post-operative 12th month bodyweights were significant (P<0.05). The results of serum parameters were non-significant statistically. As a result of this study, ovariohysterectomy in bitches have no significant early-time effect on serum total cholesterol, trigliceride, alanine aminotranspherase, alkaline phosphatase, aspartate aminotranspherase and glucose level whereas body weight gain occurs after post-operative 6th month but it can be managed by proper nutrition and exercise programmes.