This current work aims to assess the efficacy and tolerability of trabeculectomy procedure for the management of congenital glaucoma in two cats. Filtration surgery was performed using a limbus-based approach, and flap was raised smoothly to nearly limbus on sclera. Sclera-corneal trabecular block was removed both with surgical knife and microsurgery scissors. Successful result was defined as resolution of leakage with no need for additional surgery, together with maintaining intraocular pressure in the range of 5-21 mmHg with anti-glaucoma medications. In conclusion, trabeculectomy decreased intraocular pressure in cats with congenital glaucoma, there for it may proposed for treatment of related disease.