Effect of Hen Egg Yolk–Derived Low-Density Lipoprotein–Rich Fraction Supplementation in Tris Extender on the Quality and Protein Profile of Kokok Balenggek Rooster Semen during Cold Storage at 4°C

This study evaluated the effects of hen egg yolk–derived low-density lipoprotein–rich fraction supplementation in a Tris extender on the quality and sperm protein profile of Kokok Balenggek rooster semen during cold storage at 4°C. Fresh semen collected from five roosters was pooled and diluted in Tris extender containing 0%, 5%, 10%, 15%, or 20% low-density lipoprotein–rich fraction. Semen quality was assessed at 0, 24, 48, 72, 96, and 120 hours for sperm motility, viability, abnormality, and plasma membrane integrity. Motility retention and plasma membrane integrity retention were also calculated relative to 0-hour values. Sperm protein profiles were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis at 0 and 120 hours. Storage time significantly affected all semen quality parameters. Low-density lipoprotein–rich fraction supplementation improved sperm motility and plasma membrane integrity, whereas its effects on viability and abnormality were limited. The 15% low-density lipoprotein–rich fraction treatment maintained higher motility than the control at 72 and 96 hours and showed the most favorable overall preservation during storage. Plasma membrane integrity and its retention were better maintained in the 15% and 20% low-density lipoprotein–rich fraction groups during prolonged storage. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis indicated that prolonged storage reduced the complexity of the sperm protein profile, with fewer detectable bands after 120 hours. In conclusion, hen egg yolk–derived low-density lipoprotein–rich fraction supplementation in Tris extender improved the preservation of Kokok Balenggek rooster semen during cold storage, with the 15% low-density lipoprotein–rich fraction providing the most favorable overall response under the condi- tions of this study.

Cite this article as: Ananda, Ningsih, W. H., & Jaswandi. (2026). Effect of hen egg yolk–derived low-density lipoprotein–rich fraction supplementation in tris extender on the quality and protein profile of kokok balenggek rooster semen during cold storage at 4°C. Acta Veterinaria Eurasia, 52, 0050, doi:10.5152/actavet.2026.26050.